pH changes caused by bacterial growth in contaminated platelet concentrates.
نویسندگان
چکیده
While platelet concentrates are stored at room temperature, lactic and other acids are produced and the pH decreases as the buffering capacity of the plasma is exhausted. Platelet viability will be compromised if the pH decreases to pH 6.0 and below. Similarly, a pH decrease can be produced also by bacterial contamination if the organisms produce acid as an end product. Thus the determination of pH could serve as a sensitive indicator of bacterial contamination. This hypothesis was tested by us by inoculating known organisms into platelet concentrations. It was found that the pH may decrease, may remain unchanged, or, in a few cases, even increase. Visual signs of contamination could be observed but not consistently enough to be entirely dependable. Therefore, this method does not appear to detect bacterial contamination reliably in platelet concentrates.
منابع مشابه
Comparison of Diagnostic Procedures for Platelet Products Contaminated with Strains of Staphylococcus Epidermidis and Klebsiella
Abstract Background and Objective: Given that microbial contamination is the third largest cause of mortality caused blood transfusion, the examination of contamination in platelet concentrates is essential in blood transfusion centers. The purpose of this study was to achieve a rapid test for bacterial contamination of platelets concentration. Material and Methods: This laboratory ...
متن کاملEvaluation of microRNAs; mir223, mir222 and mir92a levels in the Platelet-derived microparticles in the Platelet concentrates produced by Platelet Rich Plasma method during storage
Abstract Background and Objectives Platelets release microparticles containing cellular components, including microRNAs, during storage. Assessment of these microRNAs is one of the markers for evaluation of platelet storage lesions. The aim of the present study was to evaluate the level of changes in the expression of mir-223, mir-92a and mir-222 during storage in platelets prepared by platele...
متن کاملMultiple pH measurement during storage may detect bacterially contaminated platelet concentrates.
BACKGROUND Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or consuming PCs. STUDY DESIGN AND METHODS The objective was to measure pH profi...
متن کاملPlatelet Factor 3 Based-clotting Time Assay as a Quality Marker for Long-term Storage of Platelet Concentrates
Background: Platelets rapidly lose their qualities usually after 5 day of storage. Different standard methods have been recommended to check the quality of platelets during storage which some of them show better correlation with other quality markers during storage. The purpose of this study was to demonstrate if platelet factor 3 (PF3) assay could be an indicator of storage lesion and provide ...
متن کاملThe effect of platelet concentrate storage temperature on adenine nucleotide metabolism.
The effect of platelet concentrate storage temperature (4 degrees C versus 22 degrees C) on platelet adenine nucleotide metabolism was studied. In general, levels of platelet ATP and ADP, the release reaction, and the metabolis nucleotide pool were best preserved for 72 hr by storage of concentrates at 4 degrees C. Storage of concentrates for 72 hr at 22 degrees C was occasionally associated wi...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Annals of clinical and laboratory science
دوره 15 6 شماره
صفحات -
تاریخ انتشار 1985